Nakao H, Ogli K, Yokono S, Ono J, Miyatake A
Department of Anesthesiology and Emergency Medicine, Kagawa Medical University, Japan.
Toxicol Lett. 1998 Nov 23;100-101:135-8. doi: 10.1016/s0378-4274(98)00177-5.
(1) The light-induced phosphorylation in spinach chloroplast is coupled with proton transfer. (2) We investigated the effect of volatile anesthetics (halothane, enflurane, isoflurane) on the cyclic and/or non-cyclic light-induced phosphorylation. (3) We used potassium ferricyanide as a Hill oxidant for non-cyclic phosphorylation and phenazin-methosulfate as an oxidation-reduction indicator for cyclic phosphorylation. (4) These three anesthetics inhibit the Hill reaction in light induced phosphorylation. The inhibition rate of this reaction generates concave curves with minimum values at 303 K in each of the anesthetics.
(1) 菠菜叶绿体中光诱导的磷酸化作用与质子转移相偶联。(2) 我们研究了挥发性麻醉剂（氟烷、恩氟烷、异氟烷）对循环和/或非循环光诱导磷酸化的影响。(3) 我们使用铁氰化钾作为非循环磷酸化的希尔氧化剂，使用硫酸吩嗪甲酯作为循环磷酸化的氧化还原指示剂。(4) 这三种麻醉剂抑制光诱导磷酸化中的希尔反应。该反应的抑制率在每种麻醉剂中均产生在303 K时具有最小值的凹曲线。
