Kanekatsu M, Sawada H
Laboratory of Biology, School of Liberal Arts and Sciences, Kitasato University, Sagamihara, Japan.
Biochem Mol Biol Int. 1998 Jul;45(3):561-5. doi: 10.1080/15216549800202952.
Chloroplast ribonucleoproteins (RNPs) encoded by the nuclear genome may play an important role in the post-transcriptional steps of light-induced chloroplast gene expression. To study the relationship between the synthesis of a 34 kDa ribonucleoprotein (p34) and light in spinach chloroplasts, a polyclonal antibody was raised against p34. Immunoblotting revealed that p34 synthesis was induced in a light dependent manner. In addition, levels of the large subunit of ribulose-1,5-bis phosphate carboxylase/oxygenase (Rubisco) encoded by the chloroplast genome fluctuated in relation to those of p34. These results suggest that the synthesis of p34 in response to light induces of chloroplast gene expression.
由核基因组编码的叶绿体核糖核蛋白（RNP）可能在光诱导的叶绿体基因表达的转录后步骤中发挥重要作用。为了研究菠菜叶绿体中一种34 kDa核糖核蛋白（p34）的合成与光之间的关系，制备了针对p34的多克隆抗体。免疫印迹显示p34的合成是以光依赖的方式被诱导的。此外，叶绿体基因组编码的1,5-二磷酸核酮糖羧化酶/加氧酶（Rubisco）大亚基的水平与p34的水平相关波动。这些结果表明，响应光而合成的p34诱导叶绿体基因表达。
