Poirier G M, Erlander M G
Department of Anatomy, Medical School, Birmingham University, Edgbaston, United Kingdom.
Methods. 1998 Dec;16(4):444-52. doi: 10.1006/meth.1998.0699.
The necessity of screening differentially expressed candidate genes has imposed a limit on the application of differential display to large-scale analysis of gene expression patterns. Screening candidates has indeed proven a burden because traditional screening methods require the purification of large amounts of RNA. In this article we describe an assay that allows the screening of 240 candidate genes with only 5 microg of total RNA. This assay consists of using cDNA probes synthesized from amplified RNA in differential screening and can be performed in a 96-well plate format.
