Morgan M J, Madgwick A J
Department of Orthodontics, Eastman Dental Institute for Oral Health Care Sciences, University of London, United Kingdom.
Biochem Biophys Res Commun. 1999 Feb 16;255(2):245-50. doi: 10.1006/bbrc.1999.0179.
We have determined the complete mRNA sequence of FHL3 (formerly SLIM2). We have confirmed that it is a member of the family of LIM proteins that share a similar secondary protein structure, renamed as Four-and-a-Half-LIM domain (or FHL) proteins in accordance with this structure. The "half-LIM" domain is a single zinc finger domain that may represent a subfamily of LIM domains and defines this particular family of LIM proteins. The distribution of FHL mRNA expression within a variety of murine tissues is complex. Both FHL1 and FHL3 were expressed in a number of skeletal muscles while FHL2 was expressed at high levels in cardiac muscle. Localisation of FHL3 to human chromosome 1 placed this gene in the proximity of, but not overlapping with, alleles associated with muscle diseases. FHL1 and FHL3 mRNAs were reciprocally expressed in the murine C2C12 skeletal muscle cell line and this suggested that the pattern of expression was linked to key events in myogenesis.
我们已经确定了FHL3（以前称为SLIM2）的完整mRNA序列。我们已经证实它是LIM蛋白家族的成员，这些蛋白具有相似的二级蛋白质结构，并根据此结构重新命名为四个半LIM结构域（或FHL）蛋白。“半LIM”结构域是一个单一的锌指结构域，可能代表LIM结构域的一个亚家族，并定义了这个特定的LIM蛋白家族。FHL mRNA在多种小鼠组织中的表达分布很复杂。FHL1和FHL3在许多骨骼肌中表达，而FHL2在心肌中高水平表达。FHL3定位于人类1号染色体，将该基因置于与肌肉疾病相关的等位基因附近，但不与之重叠。FHL1和FHL3 mRNA在小鼠C2C12骨骼肌细胞系中相互表达，这表明表达模式与肌生成中的关键事件有关。
