Morgan M J, Madgwick A J
Department of Orthodontics, Eastman Dental Institute for Oral Health Care Sciences, University of London, United Kingdom.
Biochem Biophys Res Commun. 1999 Feb 16;255(2):251-5. doi: 10.1006/bbrc.1999.0180.
We have determined the sequence of a novel LIM protein, termed FHL4. It was found to contain the defining secondary structural arrangement of the FHL (formerly SLIM) family of LIM proteins. This sequence was assembled using EST sequences deposited on the dbEST database and from sequencing of PCR fragments, all of which were derived from murine testis cDNA. Northern analysis of a wide range of murine tissues demonstrated that the expression of FHL4 mRNA was restricted to the testis. Using in situ hybridisation, FHL4 mRNA was found to be associated with the seminiferous epithelium and not with the interstitial tissue surrounding the seminiferous tubules. It is suggested that mFHL4 is associated with the process of spermatogenesis.
我们已经确定了一种名为FHL4的新型LIM蛋白的序列。发现它包含LIM蛋白FHL（以前称为SLIM）家族的典型二级结构排列。该序列是利用存于dbEST数据库中的EST序列以及PCR片段测序组装而成的，所有这些都来源于小鼠睾丸cDNA。对多种小鼠组织进行的Northern分析表明，FHL4 mRNA的表达仅限于睾丸。通过原位杂交发现，FHL4 mRNA与生精上皮相关，而与生精小管周围的间质组织无关。提示小鼠FHL4与精子发生过程有关。
