Chikama T, Nakamura M, Nishida T
Department of Ophthalmology, Yamaguchi University School of Medicine, 1144 Kogushi, Yamaguchi, Ube City, 755-8505, Japan.
Biochem Biophys Res Commun. 1999 Feb 24;255(3):692-7. doi: 10.1006/bbrc.1999.0267.
We previously reported that substance P and insulin-like growth factor-1 (IGF-1) synergistically facilitate corneal epithelial migration in vivo and in vitro. We investigated whether the substance P-derived tetrapeptide (phenylalanine-glycine-leucine-methionine-amide, FGLM) can up-regulate the receptors for fibronectin and cellular adhesion to fibronectin matrix in the presence or absence of IGF-1 in cultured SV-40 transformed human corneal epithelial (HCE) cells. The combination of both FGLM and IGF-1 and substance P and IGF-1 significantly increased the number of cells adhered to the fibronectin matrix and the expression of integrin alpha5. The synergistic effect of FGLM and IGF-1 on the adhesion of HCE cells to the fibronectin matrix is partly carried through up-regulation of integrin alpha5 expression in HCE cells. These results showed that the four-amino-acid sequence at the C-terminus of substance P completely mimics the substance P molecule in terms of synergism with IGF-1 on adhesion of epithelial cells to the fibronectin matrix.
我们之前报道过，P物质和胰岛素样生长因子-1（IGF-1）在体内和体外均可协同促进角膜上皮细胞迁移。我们研究了在培养的SV-40转化人角膜上皮（HCE）细胞中，P物质衍生的四肽（苯丙氨酸-甘氨酸-亮氨酸-甲硫氨酸-酰胺，FGLM）在有或无IGF-1存在的情况下，是否能上调纤连蛋白受体以及细胞与纤连蛋白基质的黏附。FGLM与IGF-1以及P物质与IGF-1的组合均显著增加了黏附于纤连蛋白基质的细胞数量以及整合素α5的表达。FGLM和IGF-1对HCE细胞与纤连蛋白基质黏附的协同作用部分是通过上调HCE细胞中整合素α5的表达来实现的。这些结果表明，P物质C末端的四氨基酸序列在与IGF-1协同促进上皮细胞与纤连蛋白基质黏附方面，完全模拟了P物质分子。
