Nakamura M, Kawahara M, Morishige N, Chikama T, Nakata K, Nishida T
Ophthalmic Research Division, Santen Pharmaceutical Co. Ltd., 8916-16 Takayama-cho, Ikoma-shi, Nara 630-0101, Japan.
Diabetologia. 2003 Jun;46(6):839-42. doi: 10.1007/s00125-003-1105-9. Epub 2003 May 22.
AIMS/HYPOTHESIS: The healing of corneal epithelial wounds is often delayed in individuals with diabetes. The effect of the combination of a substance P-derived tetrapeptide (phenylalanine-glycine-leucine-methionine amide, or FGLM-NH(2)) and insulin-like growth factor-1 (IGF-1) on corneal epithelial wound healing was investigated in rats with streptozotocin-induced diabetes.
The corneal epithelium of diabetic and non-diabetic rats was removed, and the animals were treated by the application of eye drops containing FGLM-NH(2) and IGF-1, or vehicle alone as a control, six times a day for 3 days. The area of the corneal epithelial wound was measured at various times up to 72 h after removal of the corneal epithelium.
The rate of corneal epithelial wound healing was slower in diabetic rats treated with vehicle than in non-diabetic rats. However, the rate of wound closure in diabetic rats treated with FGLM-NH(2) and IGF-1 was markedly increased compared with that in diabetic rats treated with vehicle. The wound healing process seemed similar in normal rats and in diabetic rats treated with FGLM-NH(2) and IGF-1.
CONCLUSION/INTERPRETATION: The combination of FGLM-NH(2) and IGF-1 promotes corneal epithelial wound healing in diabetic rats, suggesting that such a treatment might prove effective in humans with diabetic keratopathy.
目的/假设：糖尿病患者角膜上皮伤口愈合常常延迟。本研究在链脲佐菌素诱导的糖尿病大鼠中，探究了P物质衍生四肽（苯丙氨酸 - 甘氨酸 - 亮氨酸 - 甲硫氨酸酰胺，即FGLM - NH₂）与胰岛素样生长因子 - 1（IGF - 1）联合应用对角膜上皮伤口愈合的影响。
去除糖尿病大鼠和非糖尿病大鼠的角膜上皮，动物每天滴眼含FGLM - NH₂和IGF - 1的滴眼液或仅用赋形剂作为对照，共6次，持续3天。在去除角膜上皮后长达72小时的不同时间测量角膜上皮伤口面积。
用赋形剂处理的糖尿病大鼠角膜上皮伤口愈合速度比非糖尿病大鼠慢。然而，与用赋形剂处理的糖尿病大鼠相比，用FGLM - NH₂和IGF - 1处理的糖尿病大鼠伤口闭合速度明显加快。正常大鼠以及用FGLM - NH₂和IGF - 1处理的糖尿病大鼠的伤口愈合过程似乎相似。
结论/解读：FGLM - NH₂和IGF - 1联合应用可促进糖尿病大鼠角膜上皮伤口愈合，提示这种治疗方法可能对糖尿病性角膜病变患者有效。
