哈茨木霉内切几丁质酶基因ech42在真菌寄生中的作用。
Role of the Trichoderma harzianum endochitinase gene, ech42, in mycoparasitism.
作者信息
Carsolio C, Benhamou N, Haran S, Cortés C, Gutiérrez A, Chet I, Herrera-Estrella A
机构信息
Centro de Investigación y Estudios Avanzados, Plant Biotechnology and Genetic Engineering Unit, Irapuato, México.
出版信息
Appl Environ Microbiol. 1999 Mar;65(3):929-35. doi: 10.1128/AEM.65.3.929-935.1999.
Abstract
The role of the Trichoderma harzianum endochitinase (Ech42) in mycoparasitism was studied by genetically manipulating the gene that encodes Ech42, ech42. We constructed several transgenic T. harzianum strains carrying multiple copies of ech42 and the corresponding gene disruptants. The level of extracellular endochitinase activity when T. harzianum was grown under inducing conditions increased up to 42-fold in multicopy strains as compared with the wild type, whereas gene disruptants exhibited practically no activity. The densities of chitin labeling of Rhizoctonia solani cell walls, after interactions with gene disruptants were not statistically significantly different than the density of chitin labeling after interactions with the wild type. Finally, no major differences in the efficacies of the strains generated as biocontrol agents against R. solani or Sclerotium rolfsii were observed in greenhouse experiments.
摘要
通过对编码哈茨木霉内切几丁质酶（Ech42）的基因ech42进行基因操作，研究了Ech42在真菌寄生中的作用。我们构建了几个携带ech42多拷贝的转基因哈茨木霉菌株以及相应的基因敲除突变体。与野生型相比，在诱导条件下生长时，多拷贝菌株中哈茨木霉的细胞外内切几丁质酶活性水平提高了42倍，而基因敲除突变体几乎没有活性。与茄丝核菌细胞壁几丁质标记密度相比，与基因敲除突变体相互作用后的茄丝核菌细胞壁几丁质标记密度与与野生型相互作用后的密度没有统计学上的显著差异。最后，在温室实验中，未观察到作为生物防治剂产生的菌株对茄丝核菌或齐整小核菌的防治效果有重大差异。
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