大肠杆菌中过表达的玉米胰蛋白酶抑制剂对植物致病真菌的抑制作用
Inhibition of plant-pathogenic fungi by a corn trypsin inhibitor overexpressed in Escherichia coli.
作者信息
Chen Z Y, Brown R L, Lax A R, Cleveland T E, Russin J S
机构信息
Department of Plant Pathology and Crop Physiology, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70803, USA.
出版信息
Appl Environ Microbiol. 1999 Mar;65(3):1320-4. doi: 10.1128/AEM.65.3.1320-1324.1999.
Abstract
The cDNA of a 14-kDa trypsin inhibitor (TI) from corn was subcloned into an Escherichia coli overexpression vector. The overexpressed TI was purified based on its insolubility in urea and then refolded into the active form in vitro. This recombinant TI inhibited both conidium germination and hyphal growth of all nine plant pathogenic fungi studied, including Aspergillus flavus, Aspergillus parasiticus, and Fusarium moniliforme. The calculated 50% inhibitory concentration of TI for conidium germination ranged from 70 to more than 300 microgram/ml, and that for fungal growth ranged from 33 to 124 microgram/ml depending on the fungal species. It also inhibited A. flavus and F. moniliforme simultaneously when they were tested together. The results suggest that the corn 14-kDa TI may function in host resistance against a variety of fungal pathogens of crops.
摘要
将来自玉米的一种14千道尔顿胰蛋白酶抑制剂（TI）的cDNA亚克隆到大肠杆菌过表达载体中。基于其在尿素中的不溶性对过表达的TI进行纯化，然后在体外将其重折叠成活性形式。这种重组TI抑制了所研究的全部9种植物病原真菌的分生孢子萌发和菌丝生长，包括黄曲霉、寄生曲霉和串珠镰刀菌。计算得出TI对分生孢子萌发的50%抑制浓度范围为70至300多微克/毫升，对真菌生长的抑制浓度范围为33至124微克/毫升，具体取决于真菌种类。当同时对黄曲霉和串珠镰刀菌进行测试时，它也能同时抑制它们。结果表明，玉米14千道尔顿TI可能在宿主对多种作物真菌病原体的抗性中发挥作用。
相似文献
1
Inhibition of plant-pathogenic fungi by a corn trypsin inhibitor overexpressed in Escherichia coli.
Appl Environ Microbiol. 1999 Mar;65(3):1320-4. doi: 10.1128/AEM.65.3.1320-1324.1999.
2
A maize trypsin inhibitor (ZmTIp) with limited activity against Aspergillus flavus.
J Food Prot. 2009 Jan;72(1):185-8. doi: 10.4315/0362-028x-72.1.185.
3
PDC1, a corn defensin peptide expressed in Escherichia coli and Pichia pastoris inhibits growth of Fusarium graminearum.
Peptides. 2009 Sep;30(9):1593-9. doi: 10.1016/j.peptides.2009.05.024. Epub 2009 Jun 6.
4
Affinity purification of trypsin inhibitor with anti-Aspergillus flavus activity from cultivated and wild soybean.
Mycopathologia. 2009 Mar;167(3):163-71. doi: 10.1007/s11046-008-9160-x. Epub 2008 Oct 1.
5
Inhibition of growth of Aspergillus flavus and fungal alpha-amylases by a lectin-like protein from Lablab purpureus.
Mol Plant Microbe Interact. 2001 Aug;14(8):955-61. doi: 10.1094/MPMI.2001.14.8.955.
6
Survival of Aspergillus flavus and Fusarium moniliforme in high-moisture corn stored under modified atmospheres.
Appl Microbiol. 1975 Oct;30(4):592-5. doi: 10.1128/am.30.4.592-595.1975.
7
A study on trypsin, Aspergillus flavus and Bacillus sp. protease inhibitory activity in Cassia tora (L.) syn Senna tora (L.) Roxb. seed extract.
BMC Complement Altern Med. 2011 Jul 12;11:56. doi: 10.1186/1472-6882-11-56.
8
Zeamatin inhibits trypsin and alpha-amylase activities.
Appl Environ Microbiol. 2001 May;67(5):2365-6. doi: 10.1128/AEM.67.5.2365-2366.2001.
9
A protective endophyte of maize: Acremonium zeae antibiotics inhibitory to Aspergillus flavus and Fusarium verticillioides.
Mycol Res. 2005 May;109(Pt 5):610-8.
10
In vitro studies on the potential for biological control of Aspergillus flavus and Fusarium moniliforme by Trichoderma species. 1. Macroscopical and microscopical observations of fungal interactions.
Mycopathologia. 1997;139(2):115-21. doi: 10.1023/a:1006867907885.
引用本文的文献
1
Antifungal effect and some properties of cell-free supernatants of two Bacillus subtilis isolates against Fusarium verticillioides.
Braz J Microbiol. 2024 Sep;55(3):2527-2538. doi: 10.1007/s42770-024-01414-x. Epub 2024 Jun 11.
2
RNAi-Mediated Suppression of Promotes Salt Stress Tolerance in Rice.
Int J Mol Sci. 2024 Jan 20;25(2):1284. doi: 10.3390/ijms25021284.
3
Tandem Multimerization Can Enhance the Structural Homogeneity and Antifungal Activity of the Silkworm Protease Inhibitor BmSPI39.
Cells. 2023 Feb 22;12(5):693. doi: 10.3390/cells12050693.
4
Synthesis and accumulation of amylase-trypsin inhibitors and changes in carbohydrate profile during grain development of bread wheat (Triticum aestivum L.).
BMC Plant Biol. 2021 Feb 24;21(1):113. doi: 10.1186/s12870-021-02886-x.
5
Heterologous Expression of PKPI and Pin1 Proteinase Inhibitors Enhances Plant Fitness and Broad-Spectrum Resistance to Biotic Threats.
Front Plant Sci. 2020 Apr 30;11:461. doi: 10.3389/fpls.2020.00461. eCollection 2020.
6
A fungal effector and a rice NLR protein have antagonistic effects on a Bowman-Birk trypsin inhibitor.
Plant Biotechnol J. 2020 Nov;18(11):2354-2363. doi: 10.1111/pbi.13400. Epub 2020 Jul 4.
7
Comparative Histological and Transcriptional Analysis of Maize Kernels Infected with and .
Front Plant Sci. 2017 Dec 6;8:2075. doi: 10.3389/fpls.2017.02075. eCollection 2017.
8
Protein profiles reveal diverse responsive signaling pathways in kernels of two maize inbred lines with contrasting drought sensitivity.
Int J Mol Sci. 2014 Oct 20;15(10):18892-918. doi: 10.3390/ijms151018892.
9
Interactions outside the proteinase-binding loop contribute significantly to the inhibition of activated coagulation factor XII by its canonical inhibitor from corn.
J Biol Chem. 2014 May 16;289(20):14109-20. doi: 10.1074/jbc.M114.553735. Epub 2014 Apr 4.
10
Proteomics to identify resistance factors in corn-a review.
Mycotoxin Res. 2006 Mar;22(1):22-6. doi: 10.1007/BF02954553.
本文引用的文献
1
Resistance to Aspergillus flavus in Corn Kernels Is Associated with a 14-kDa Protein.
Phytopathology. 1998 Apr;88(4):276-81. doi: 10.1094/PHYTO.1998.88.4.276.
2
Synergistic Enhancement of the Antifungal Activity of Wheat and Barley Thionins by Radish and Oilseed Rape 2S Albumins and by Barley Trypsin Inhibitors.
Plant Physiol. 1993 Dec;103(4):1311-1319. doi: 10.1104/pp.103.4.1311.
3
Cysteine protease inhibitor from pearl millet: a new class of antifungal protein.
Biochem Biophys Res Commun. 1998 May 19;246(2):382-7. doi: 10.1006/bbrc.1998.8625.
4
Cloning and overexpression of two cDNAs encoding the low-CO2-inducible chloroplast envelope protein LIP-36 from Chlamydomonas reinhardtii.
Plant Physiol. 1997 May;114(1):265-73. doi: 10.1104/pp.114.1.265.
5
Levels of fumonisin B1 in corn naturally contaminated with aflatoxins.
Food Chem Toxicol. 1993 Dec;31(12):995-8. doi: 10.1016/0278-6915(93)90009-n.
6
Natural plant enzyme inhibitors. Characterization of an unusual alpha-amylase/trypsin inhibitor from ragi (Eleusine coracana Geartn.).
Biochem J. 1981 Jan 1;193(1):29-36. doi: 10.1042/bj1930029.
7
Hageman factor fragment inhibitor in corn seeds: purification and characterization.
Thromb Res. 1980 Oct 15;20(2):149-62. doi: 10.1016/0049-3848(80)90381-3.
8
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
9
Refolding of recombinant proteins.
Methods Enzymol. 1990;185:187-95. doi: 10.1016/0076-6879(90)85018-j.
10
Isolation and characterization of a 22 kDa protein with antifungal properties from maize seeds.
Biochem Biophys Res Commun. 1992 Jan 15;182(1):1-5. doi: 10.1016/s0006-291x(05)80103-2.
Zotero 插件