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木霉属真菌对黄曲霉和串珠镰刀菌进行生物防治潜力的体外研究。1. 真菌相互作用的宏观和微观观察。

In vitro studies on the potential for biological control of Aspergillus flavus and Fusarium moniliforme by Trichoderma species. 1. Macroscopical and microscopical observations of fungal interactions.

作者信息

Calistru C, McLean M, Berjak P

机构信息

Department of Biology, University of Natal, Durban, South Africa.

出版信息

Mycopathologia. 1997;139(2):115-21. doi: 10.1023/a:1006867907885.

DOI:10.1023/a:1006867907885
PMID:9565501
Abstract

The possible biological control of the seed-associated fungi, Aspergillus flavus and Fusarium moniliforme by Trichoderma species was investigated. A study of the fungal growth in dual cultures revealed that four of nine isolates of two Trichoderma spp. (T. harzianum and T. viride) inhibited fungal growth. A scanning electron microscopical investigation of fungal interactions demonstrated no obvious hyphae penetration of A. flavus or F. moniliforme by Trichoderma spp. Morphological alteration of Fusarium hyphae with pronounced collapse and the production of aberrant conidial heads and microheads by A. flavus were observed. The results suggest that mycoparasitism is not the mechanism involved in the inhibitory interaction of either A. flavus or F. moniliforme with Trichoderma spp.

摘要

研究了木霉菌对与种子相关的真菌黄曲霉和串珠镰刀菌的可能生物防治作用。对双培养物中真菌生长的研究表明,两种木霉菌(哈茨木霉和绿色木霉)的九个分离株中有四个抑制了真菌生长。对真菌相互作用的扫描电子显微镜研究表明,木霉菌种未明显穿透黄曲霉或串珠镰刀菌的菌丝。观察到镰刀菌菌丝形态改变,明显塌陷,黄曲霉产生异常的分生孢子头和微小头。结果表明,互寄生不是黄曲霉或串珠镰刀菌与木霉菌种抑制相互作用的机制。

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