Yang Liming, Jiang Tingbo, Fountain Jake C, Scully Brian T, Lee Robert D, Kemerait Robert C, Chen Sixue, Guo Baozhu
USDA-ARS, Crop Protection and Management Research Unit, Tifton, GA 31793, USA.
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China.
Int J Mol Sci. 2014 Oct 20;15(10):18892-918. doi: 10.3390/ijms151018892.
Drought stress is a major factor that contributes to disease susceptibility and yield loss in agricultural crops. To identify drought responsive proteins and explore metabolic pathways involved in maize tolerance to drought stress, two maize lines (B73 and Lo964) with contrasting drought sensitivity were examined. The treatments of drought and well water were applied at 14 days after pollination (DAP), and protein profiles were investigated in developing kernels (35 DAP) using iTRAQ (isobaric tags for relative and absolute quantitation). Proteomic analysis showed that 70 and 36 proteins were significantly altered in their expression under drought treatments in B73 and Lo964, respectively. The numbers and levels of differentially expressed proteins were generally higher in the sensitive genotype, B73, implying an increased sensitivity to drought given the function of the observed differentially expressed proteins, such as redox homeostasis, cell rescue/defense, hormone regulation and protein biosynthesis and degradation. Lo964 possessed a more stable status with fewer differentially expressed proteins. However, B73 seems to rapidly initiate signaling pathways in response to drought through adjusting diverse defense pathways. These changes in protein expression allow for the production of a drought stress-responsive network in maize kernels.
干旱胁迫是导致农作物易患病和产量损失的主要因素。为了鉴定干旱响应蛋白并探索玉米耐旱性所涉及的代谢途径，研究了两个对干旱敏感性不同的玉米品系（B73和Lo964）。在授粉后14天（DAP）进行干旱和正常浇水处理，并使用iTRAQ（相对和绝对定量的等压标签）对发育中的籽粒（35 DAP）的蛋白质谱进行研究。蛋白质组学分析表明，在干旱处理下，B73和Lo964中分别有70和36种蛋白质的表达发生了显著变化。敏感基因型B73中差异表达蛋白质的数量和水平通常更高，鉴于所观察到的差异表达蛋白质的功能，如氧化还原稳态、细胞拯救/防御、激素调节以及蛋白质生物合成和降解，这意味着对干旱的敏感性增加。Lo964具有更稳定的状态，差异表达蛋白质较少。然而，B73似乎通过调节多种防御途径来快速启动响应干旱的信号通路。这些蛋白质表达的变化促成了玉米籽粒中干旱胁迫响应网络的形成。
