从胚胎细胞原代培养物中分离果蝇成肌细胞并进行部分特性分析。
Isolation and partial characterization of Drosophila myoblasts from primary cultures of embryonic cells.
作者信息
Bernstein S I, Fyrberg E A, Donady J J
出版信息
J Cell Biol. 1978 Sep;78(3):856-65. doi: 10.1083/jcb.78.3.856.
Abstract
We describe a method for preparing highly enriched cultures of Drosophila myoblasts from a heterogeneous cell population derived from gastrulating embryos. Enriched cultures are prepared by plating this heterogeneous population of cells in medium from which much of the free calcium is chelated by ethylene glycol-bis(beta-aminoethyl ether)N,N,N',N'-tetraacetate (EGTA). Adhesion of myoblasts to tissue culture plastic is better than that of other cell types when plated in this medium. Data concerning cell identity, timing of S phase, and fusion kinetics document the degree of enrichment for myogenic cells and illustrate their synchronous differentiation in vitro.
摘要
我们描述了一种从原肠胚形成期胚胎来源的异质细胞群体中制备高度富集的果蝇成肌细胞培养物的方法。通过将这种异质细胞群体接种在一种培养基中来制备富集培养物，该培养基中的大部分游离钙被乙二醇双（β-氨基乙醚）N，N，N'，N'-四乙酸（EGTA）螯合。当接种在这种培养基中时，成肌细胞与组织培养塑料的粘附性优于其他细胞类型。有关细胞身份、S期时间和融合动力学的数据记录了成肌细胞的富集程度，并说明了它们在体外的同步分化。
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