Misao R, Nakanishi Y, Sun W S, Fujimoto J, Iwagaki S, Hirose R, Tamaya T
Department of Obstetrics and Gynecology, Gifu University School of Medicine, Gifu City, Japan.
Mol Hum Reprod. 1999 Jan;5(1):17-21. doi: 10.1093/molehr/5.1.17.
To investigate the role of oestrogen receptor beta (ERbeta) in the function of human ovarian corpus luteum, the levels of luteal ERalpha and ERbeta mRNA were determined using competitive reverse transcription-polymerase chain reaction-Southern blot analysis. The expression of ERalpha and ERbeta mRNA was detected in all luteal samples analysed. Luteal ERalpha and ERbeta mRNA levels were significantly lower (P<0.01 and P<0.05 respectively) at the late secretory phase than those at the early and mid-secretory phases of the endometrium. The ratio of ERalpha to ERbeta mRNA levels showed no change during the secretory phase of the endometrium. This study demonstrates that ERbeta is co-expressed with ERalpha in human corpus luteum and is likely to play a biological role in the regulation of steroidal action of the corpus luteum with ERalpha.
为研究雌激素受体β（ERβ）在人卵巢黄体功能中的作用，采用竞争性逆转录-聚合酶链反应- Southern印迹分析法测定黄体中ERα和ERβ mRNA的水平。在所分析的所有黄体样本中均检测到ERα和ERβ mRNA的表达。在子宫内膜分泌晚期，黄体ERα和ERβ mRNA水平显著低于分泌早期和中期（分别为P<0.01和P<0.05）。在子宫内膜分泌期，ERα与ERβ mRNA水平的比值无变化。本研究表明，ERβ与人黄体中的ERα共同表达，可能与ERα一起在黄体甾体作用的调节中发挥生物学作用。
