Misao R, Nakanishi Y, Fujimoto J, Tamaya T
Department of Obstetrics and Gynecology, Gifu University School of Medicine, Gifu, Japan.
Horm Res. 1998 Sep;50(3):155-9. doi: 10.1159/000023265.
To understand the biology of sex steroids in the human corpus luteum, the expression of estrogen receptor alpha, progesterone receptor, and androgen receptor mRNA levels was determined by semiquantitative reverse-transcription polymerase chain reaction-Southern blot analysis. Expression of all receptor mRNAs was detected in all samples analyzed. Each steroid receptor mRNA level was significantly lower (p < 0.05) during the late secretory phase than that during the early or the mid-secretory phase of the endometrium. These findings support the concept of a local role for sex steroids in modulating the function and life span of the human corpus luteum.
为了解人类黄体中性类固醇的生物学特性，通过半定量逆转录聚合酶链反应- Southern印迹分析来测定雌激素受体α、孕激素受体和雄激素受体mRNA水平的表达。在所有分析的样本中均检测到所有受体mRNA的表达。在子宫内膜分泌晚期，每种类固醇受体mRNA水平均显著低于分泌早期或中期（p < 0.05）。这些发现支持了性类固醇在调节人类黄体功能和寿命方面具有局部作用的观点。
