Makeyev E V, Kolb V A, Spirin A S
Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region.
FEBS Lett. 1999 Feb 12;444(2-3):177-80. doi: 10.1016/s0014-5793(99)00055-1.
A novel cloning-independent strategy has been developed to generate a combinatorial library of PCR fragments encoding a murine single-chain antibody repertoire and express it directly in a cell-free system. The new approach provides an effective alternative to the techniques involving in vivo procedures of preparation and handling large libraries of antibodies. The possible use of the described strategy in the ribosome display is discussed.
已开发出一种新的不依赖克隆的策略，用于生成编码鼠单链抗体库的PCR片段组合文库，并直接在无细胞系统中进行表达。这种新方法为涉及体内制备和处理大型抗体库程序的技术提供了一种有效的替代方案。文中还讨论了所描述策略在核糖体展示中的可能应用。
