反义寡核苷酸与tRNA（苯丙氨酸）3'部分的杂交。
Hybridization of antisense oligonucleotides with the 3'part of tRNA(Phe).
作者信息
Petyuk V A, Zenkova M A, Giege R, Vlassov V V
机构信息
Institute of Bioorganic Chemistry, Russian Academy of Sciences, Novosibirsk.
出版信息
FEBS Lett. 1999 Feb 12;444(2-3):217-21. doi: 10.1016/s0014-5793(99)00063-0.
The interaction of antisense oligodeoxyribonucleotides with yeast tRNA(Phe) was investigated. 14-15-mers complementary to the 3'-terminal sequence including the ACCA end bind to the tRNA under physiological conditions. At low oligonucleotide concentrations the binding occurs at the unique complementary site. At higher oligonucleotide concentrations, the second oligonucleotide molecule binds to the complex due to non-perfect duplex formation in the T-loop stabilized by stacking between the two bound oligonucleotides. In these complexes the acceptor stem is open and the 5'-terminal sequence of the tRNA is accessible for binding of a complementary oligonucleotide. The results prove that the efficient binding of oligonucleotides to the 3'-terminal sequence of the tRNA occurs through initial binding to the single-stranded sequence ACCA followed by invasion in the acceptor stem and strand displacement.
研究了反义寡脱氧核糖核苷酸与酵母苯丙氨酸转运核糖核酸（tRNA(Phe)）的相互作用。与包括ACCA末端在内的3'-末端序列互补的14 - 15聚体在生理条件下与tRNA结合。在低寡核苷酸浓度下，结合发生在独特的互补位点。在较高寡核苷酸浓度下，由于两个结合的寡核苷酸之间的堆积作用使T环中形成非完美双链体，第二个寡核苷酸分子会与该复合物结合。在这些复合物中，受体茎是开放的，tRNA的5'-末端序列可用于结合互补寡核苷酸。结果证明，寡核苷酸与tRNA的3'-末端序列的有效结合是通过首先与单链序列ACCA结合，然后侵入受体茎并进行链置换实现的。
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