用与双咪唑结构缀合的寡核苷酸对酵母苯丙氨酸转运RNA进行序列特异性切割。
Sequence-specific cleavage of yeast tRNA(Phe) with oligonucleotides conjugated to a diimidazole construct.
作者信息
Vlassov V, Abramova T, Godovikova T, Giege R, Silnikov V
机构信息
Institute of Bioorganic Chemistry, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
出版信息
Antisense Nucleic Acid Drug Dev. 1997 Feb;7(1):39-42. doi: 10.1089/oli.1.1997.7.39.
Oligonucleotide derivatives conjugated to a chemical construction with two histamine residues imitating the catalytic center of ribonuclease A have been synthesized. In experiments with the conjugates complementary to the 3'-end and to the variable loop and the T loop of yeast tRNA(Phe), it was shown that the compounds can accomplish sequence-specific cleavage of the target RNA in physiologic conditions.
已合成与具有两个组胺残基的化学结构共轭的寡核苷酸衍生物，该化学结构模仿核糖核酸酶A的催化中心。在用与酵母tRNA（苯丙氨酸）的3'-末端、可变环和T环互补的共轭物进行的实验中，结果表明这些化合物能够在生理条件下完成对靶RNA的序列特异性切割。
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