一种用于在聚乙烯亚胺（PEI）纤维素薄层上分离单磷酸核苷的改进方法。
An improved separation procedure for nucleoside monophosphates on polyethyleneimine-(PEI-)cellulose thin layers.
作者信息
Gupta R C, Randerath E, Randerath K
出版信息
Nucleic Acids Res. 1976 Nov;3(11):2915-21. doi: 10.1093/nar/3.11.2915.
Abstract
A procedure is described for the two-dimensional separation of the 4 major and 16 modified nucleoside-(5') monophosphates on anion-exchange thin layers of polyethyleneimine- (PEI-)cellulose. The method, which is simple and less time-consuming than existing partition chromatographic methods, may be used for the identification of 5'-termini of RNA and RNA fragments.
摘要
本文描述了一种在聚乙烯亚胺（PEI）-纤维素阴离子交换薄层上对4种主要核苷-（5'）单磷酸和16种修饰核苷-（5'）单磷酸进行二维分离的方法。该方法简单，且比现有的分配色谱方法耗时少，可用于鉴定RNA及RNA片段的5'-末端。
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