一种通过低速离心大量制备脑微粒体的方法。
A procedure for the bulk preparation of brain microsomes with low speed centrifugation.
作者信息
Guarnieri M, Finnegan A, DeMarchena O
出版信息
Prep Biochem. 1976;6(6):489-501. doi: 10.1080/00327487608069131.
PMID:1005360
Abstract
A method is described for the rapid isolation of microsomes from brains of various species. The method depends on the interaction of calcium with microsomes. The procedure is limited because calcium inhibits certain microsomal enzymes. Magnesium and manganese may be substituted for calcium. However, the substitutions decrease the reliability and efficiency of the procedure.
摘要
本文描述了一种从不同物种大脑中快速分离微粒体的方法。该方法依赖于钙与微粒体的相互作用。该程序存在局限性，因为钙会抑制某些微粒体酶。镁和锰可以替代钙。然而，这种替代会降低该程序的可靠性和效率。
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