Tamai Y, Araki S, Komai Y, Satake M
J Neurosci Res. 1975;1(2):161-82. doi: 10.1002/jnr.490010208.
The procedure for bulk preparation of nerve-cell perikarya from pig brain-stem is briefly described. This method has definite merits with regard to the preservation of the intracellular structures as well as the preservation of the processes. The chemical composition of the isolated nerve-cell perikarya is presented. The nerve-cell perikarya are characterized by the absence of cerebroside and sulfatide. Diffuse distribution of ganglioside on the cell surface is suggested. The lipid content of the nerve-cell perikarya isolated by different methods is discussed. Lipid composition of the invertebrate nervous tissue is discussed. The submicrosomal membranes of rat brain are isolated, and their chemical and morphological properties are discussed. Ribosome-free membranes are characterized by the high content of glycolipids including gangliosides, as well as by the high activity of Na, K-ATPase, whereas ribosome-bound membranes are characterized by the absence of glycolipids, as well as by the low activity of Na, K-ATPase. The molecular organizations of lipid in submicrosomal membranes are demonstrated.
简要描述了从猪脑干批量制备神经细胞胞体的过程。该方法在保存细胞内结构以及保存突起方面具有一定优点。给出了分离出的神经细胞胞体的化学成分。神经细胞胞体的特征是不存在脑苷脂和硫脂。提示神经节苷脂在细胞表面呈弥散分布。讨论了用不同方法分离的神经细胞胞体的脂质含量。讨论了无脊椎动物神经组织的脂质组成。分离了大鼠脑的亚微粒体膜，并讨论了其化学和形态学特性。无核糖体膜的特征是包括神经节苷脂在内的糖脂含量高，以及钠钾 -ATP 酶活性高，而结合核糖体的膜的特征是不存在糖脂，以及钠钾 -ATP 酶活性低。展示了亚微粒体膜中脂质的分子组织。
