去氧皮质酮、戈德布拉特和自发性高血压大鼠肾髓质组织中前列腺素E2的产生

Prostaglandin E2 production by renomedullary tissue of DOCA, Goldblatt and spontaneously hypertensive rats.

作者信息

Stygles V G, Reinke D A, Hook J B

出版信息

Res Commun Chem Pathol Pharmacol. 1976 Dec;15(4):753-65.

Abstract

The realtionship between in vitro prostaglandin production by renal medullary tissue and hypertension was investigated in three models of hypertensive rat, i.e., DOCA, Goldblatt and SHR. Medullary minces were incubated for 30 minutes. Following incubation tissue PGE2 concentration was quantified using mass fragmentography. Media PGE2 concentrations were quantified using radioimmunoassay. Concentrations of PGE2 in tissue from Goldblatts and SHRs were significantly less than normotensive controls. The concentration of PGE2 in media after incubating SHR tissue was significantly less than normotensives. The data suggest that alterations in PGE2 metabolism are similar in Goldblatt and SHR hypertension.

摘要

在三种高血压大鼠模型（即去氧皮质酮乙酸盐（DOCA）、戈德布拉特（Goldblatt）和自发性高血压大鼠（SHR））中，研究了肾髓质组织体外前列腺素生成与高血压之间的关系。将髓质切碎物孵育30分钟。孵育后，使用质谱碎片分析法对组织中前列腺素E2（PGE2）浓度进行定量。使用放射免疫分析法对培养基中PGE2浓度进行定量。戈德布拉特模型大鼠和自发性高血压大鼠组织中的PGE2浓度显著低于正常血压对照组。自发性高血压大鼠组织孵育后培养基中PGE2浓度显著低于正常血压大鼠。数据表明，戈德布拉特模型大鼠高血压和自发性高血压大鼠高血压中PGE2代谢的改变相似。