Roessner A, Uchida Y, Van Husen N, Gerlach U, Witting U
Res Exp Med (Berl). 1976 Nov 25;169(1):9-20. doi: 10.1007/BF01851335.
In the present study an ultrastructural morphometric analysis of the rat liver 1, 3 and 7 days after bile duct ligation was performed. Corresponding biochemical investigations included the determination of the cytochrom P 450 contents in the microsomal fraction. In the serum of the rats the activities of GOT and GPT were determined. The quantitative results have shown that the decreased P 450 contents of the microsomal fraction coincided with a considerable increasement of the surface of the smooth endoplasmic reticulum. The smooth endoplasmic reticulum was significantly increased from 1.83 m2/cm3 hepatocytes to 3.49 m2/cm3 hepatocytes after 3 days and remained on this level after 7 days. These results may be considered as quantitative evidence for the term hyperplastic, hypoactive endoplasmic reticulum. - As a second important result our quantitative investigations have shown that the increasement of GLDH activity in the serum especially 1 day after ligation of the bile duct coincided with a signficantly increased mitochondrial volume at this time.
在本研究中，对胆管结扎术后1天、3天和7天的大鼠肝脏进行了超微结构形态计量分析。相应的生化研究包括测定微粒体部分的细胞色素P 450含量。测定了大鼠血清中谷草转氨酶（GOT）和谷丙转氨酶（GPT）的活性。定量结果表明，微粒体部分细胞色素P 450含量的降低与滑面内质网表面积的显著增加相吻合。滑面内质网在3天后从1.83平方米/立方厘米肝细胞显著增加到3.49平方米/立方厘米肝细胞，并在7天后维持在这一水平。这些结果可被视为内质网增生、活性降低这一术语的定量证据。作为第二个重要结果，我们的定量研究表明，血清中谷氨酸脱氢酶（GLDH）活性的增加，尤其是在胆管结扎后1天，与此时线粒体体积的显著增加相吻合。
