Nishiura S, Koga A, Yanagisawa J
Department of Surgery I, Kyushu University, Faculty of Medicine, Fukuoka, Japan.
Exp Mol Pathol. 1988 Aug;49(1):62-74. doi: 10.1016/0014-4800(88)90021-4.
The effects of biliary obstruction and drainage on the hepatic microsomal mixed function oxidase system were studied in rats. Bile duct obstruction produced a significant reduction in the hepatic cytochrome P-450 dependent mixed function oxidase system. After release of the bile duct obstruction, the reduction in microsomal enzymes was practically reversible; however, the process of recovery was slow and differed with the microsomal enzymes in question. Increases in cytochrome b5 content and NADH-cytochrome b5 reductase activity were slower than increases in cytochrome P-450 content and NADPH-cytochrome c reductase activity. Aniline hydroxylase activity increased more rapidly and corresponded to cytochrome P-450 contents more so than did the aminopyrine demethylase activity. After the release of bile duct obstruction, however, the bile acids which had accumulated in the liver during cholestasis were reduced rapidly, to a normal range. These results suggests that there is a discrepancy between reductions in hepatic bile acids and those in the hepatic microsomal mixed function oxidase system after biliary decompression.
在大鼠中研究了胆道梗阻及引流对肝微粒体混合功能氧化酶系统的影响。胆管梗阻使肝脏中细胞色素P - 450依赖的混合功能氧化酶系统显著降低。胆管梗阻解除后，微粒体酶的降低实际上是可逆的；然而，恢复过程缓慢，且因所涉及的微粒体酶不同而有所差异。细胞色素b5含量和NADH - 细胞色素b5还原酶活性的增加比细胞色素P - 450含量和NADPH - 细胞色素c还原酶活性的增加要慢。苯胺羟化酶活性增加得更快，并且与细胞色素P - 450含量的对应关系比氨基比林脱甲基酶活性更强。然而，胆管梗阻解除后，胆汁淤积期间肝脏中积累的胆汁酸迅速减少至正常范围。这些结果表明，胆道减压后肝脏胆汁酸的减少与肝微粒体混合功能氧化酶系统的减少之间存在差异。
