Soulier J P, Patereau C
Rev Fr Transfus Immunohematol. 1976 Sep;19(3):431-48. doi: 10.1016/s0338-4535(76)80019-0.
A platelet indirect radio-active Coombs test has been described. The technique for purification and labelling the antiglobulin has been precised. This test allows the typing of platelets in the PLA system and the study of sera from mothers of thrombocytopenic child. As examples, four families of neonatal thrombocytopenia are reported, with PLA1 negative mother. In the serum of three of these mothers, we could demonstrate anti-PLA antibodies in spite of a negative platelet complement fixation. This test has many advantages compared to other tests such as platelet complement fixation, assay for blocking antibodies or antiglobulin consumption: it gives objective and quantitative results and is highly reproducible, anticomplementary serum may be tested. It has enabled us to select PLA1 negative donors for exsanguino-transfusions of thrombocytopenic children born from PLA1 negative mothers.
已描述了一种血小板间接放射性库姆斯试验。抗球蛋白纯化和标记技术已明确。该试验可用于PLA系统中血小板的分型以及对血小板减少症患儿母亲血清的研究。作为示例，报告了四个新生儿血小板减少症家庭，其母亲为PLA1阴性。在其中三位母亲的血清中，尽管血小板补体结合试验为阴性，但我们仍能检测到抗PLA抗体。与其他试验如血小板补体结合试验、封闭抗体检测或抗球蛋白消耗试验相比，该试验具有许多优点：它能给出客观和定量的结果且重复性高，可检测抗补体血清。它使我们能够为PLA1阴性母亲所生的血小板减少症患儿选择PLA1阴性献血者进行放血输血。
