Soulier J P, Patereau C, Drouet J
Vox Sang. 1975;29(4):253-68. doi: 10.1111/j.1423-0410.1975.tb00506.x.
A platelet indirect radioactive Coombs test (PIRC) has been described. The technique for purification and labelling the antiglobulin has been precised. This test allowed the typing of platelets in the PLA system by using an absorbed serum from a mother of a thrombocytopenic child. Six other families of neonatal thrombocytopenias were tested. In three of them, the mothers were found PLA1 negative (PLA2, PLA2). Among a panel of 93 platelets, two (0.022) were found PLA1, negative. This PIRC test has many advantages compared to other tests such as platelet complement fixation, assay for blocking antibodies or antiglobulin consumption: it gives objective and quantitative results and is highly reproducible; anticomplementary serum may be tested.
已描述了血小板间接放射性库姆斯试验（PIRC）。抗球蛋白纯化和标记技术已明确。该试验通过使用来自血小板减少症患儿母亲的吸收血清对PLA系统中的血小板进行分型。对另外六个新生儿血小板减少症家庭进行了检测。其中三个家庭的母亲被发现为PLA1阴性（PLA2、PLA2）。在一组93个血小板中，发现两个（0.022）为PLA1阴性。与其他试验如血小板补体结合试验、封闭抗体检测或抗球蛋白消耗试验相比，该PIRC试验有许多优点：它能给出客观和定量的结果，且重复性高；可检测抗补体血清。
