Medenica R, Maurice P, Cruchaud A, Wyss M
Schweiz Med Wochenschr. 1976 Oct 2;106(40):1369-70.
A new method called "repetitive filtration leukopheresis" is described for granulocyte transfusion therapy. 23 patients received a total of 91 transfusions. All patients presented neutropenia of less than 300/mm3 and various kinds of infection resistant to antibiotic therapy. A favorable result was observed in 18 cases following these transfusions, which did not produce the secondary effects noted by others (chills, rash, fever, dyspnea). It was felt that this remarkable tolerance was a result of the collection procedure (elution at pH 7.4 ommission of centrifugation, thus securing the functional integrity of the cells). This impression was confirmed by the results of a battery of tests performed on the collected granulocytes, which included evaluation of their phagocytic and bacteriolytic functions and of their ability to break down a phagocytized antigen, together with measurements of lysosomial enzymes released in the supernatant.
本文描述了一种名为“重复过滤白细胞去除术”的粒细胞输血治疗新方法。23例患者共接受了91次输血。所有患者均出现中性粒细胞减少症，中性粒细胞计数低于300/mm³，并伴有对抗生素治疗耐药的各种感染。这些输血后，18例患者取得了良好效果，且未产生其他人所指出的副作用（寒战、皮疹、发热、呼吸困难）。据认为，这种显著的耐受性是采集程序的结果（在pH 7.4时洗脱，省略离心，从而确保细胞的功能完整性）。对采集的粒细胞进行的一系列测试结果证实了这一印象，这些测试包括评估粒细胞的吞噬和溶菌功能、分解吞噬抗原的能力，以及测量上清液中释放的溶酶体酶。
