Aarestrup F M, Jensen N E
Danish Veterinary Laboratory, Copenhagen, Denmark.
Vet Microbiol. 1999 Feb 12;64(4):299-305. doi: 10.1016/s0378-1135(97)00119-3.
This study was conducted to compare the applicability of three different media in sensitivity testing of Actinobacillus pleuropneumoniae by means of MIC and tablet diffusion tests. The media used were: modified PPLO agar, chocolatized Mueller-Hinton-II and Columbia agar supplemented with NAD. Seven antimicrobial agents were tested: ceftiofur, enrofloxacin, penicillin, spectinomycin, tiamulin, trimethoprim + sulfadiazine and tylosin, against 40 randomly selected A. pleuropneumoniae isolates. In general, good agreement was found between results obtained with all combinations of media, most antimicrobials tested and the two-test systems. Some variations between media were observed for spectinomycin, tiamulin and tylosin. For ceftiofur and trimethoprim + sulfadiazine some isolates with low MIC-values were classified as resistant using tablet diffusion, indicating that the break points of resistance for these antimicrobials using the tablet diffusion tests need adjustment. Using current break points for resistance with MIC-determinations, all isolates tested susceptible to ceftiofur, enrofloxacin, penicillin, tiamulin and trimethoprim + sulfadiazine. A larger number of isolates tested resistant to spectinomycin and tylosin on all three media using both MIC determinations and tablet diffusion.
本研究旨在通过最低抑菌浓度（MIC）和纸片扩散试验比较三种不同培养基在胸膜肺炎放线杆菌敏感性试验中的适用性。所用培养基为：改良PPLO琼脂、巧克力化的Mueller-Hinton-II琼脂和补充了烟酰胺腺嘌呤二核苷酸（NAD）的哥伦比亚琼脂。对40株随机选取的胸膜肺炎放线杆菌分离株测试了七种抗菌剂：头孢噻呋、恩诺沙星、青霉素、壮观霉素、泰妙菌素、甲氧苄啶 + 磺胺嘧啶和泰乐菌素。总体而言，在所有培养基组合、大多数测试抗菌剂和两种测试系统所获得的结果之间发现了良好的一致性。对于壮观霉素、泰妙菌素和泰乐菌素，观察到不同培养基之间存在一些差异。对于头孢噻呋和甲氧苄啶 + 磺胺嘧啶，一些MIC值较低的分离株在纸片扩散试验中被判定为耐药，这表明使用纸片扩散试验时这些抗菌剂的耐药断点需要调整。使用当前MIC测定法的耐药断点，所有测试分离株对头孢噻呋、恩诺沙星、青霉素、泰妙菌素和甲氧苄啶 + 磺胺嘧啶均敏感。使用MIC测定法和纸片扩散法，在所有三种培养基上测试的大量分离株对壮观霉素和泰乐菌素耐药。
