Busch D H, Pamer E G
Section of Infectious Diseases, Yale University, New Haven CT 06520, USA.
Immunol Lett. 1999 Jan;65(1-2):93-8. doi: 10.1016/s0165-2478(98)00130-8.
Infection of mice with Listeria monocytogenes results in a robust T lymphocyte response that clears the pathogen and provides long term immunity from reinfection. The number of splenic CD4+ and CD8+ T cells and natural killer cells increases during primary and recall infection with L. monocytogenes, however the proportional increase is greatest for CD8+ T cells. The proportion of CD8 T cells expressing low levels of CD62L, a sign of activation, was increased among immune splenocytes, suggesting a substantial expansion of L. monocytogenes specific CTL. Analysis of CTL specific for the immunodominant LLO 91-99 epitope showed that essentially all were CD62Llo during the primary response, but that many upregulated expression of CD62L during the memory phase. Interestingly, the antigen specificity of nearly all additional CD62Llo CTL detected in spleens during recall L. monocytogenes infection can be accounted for with MHC class I tetramers complexed with four different epitopes. These studies demonstrate the complex T lymphocyte dynamics during infection with intracellular pathogens.
用单核细胞增生李斯特菌感染小鼠会引发强烈的T淋巴细胞反应，该反应可清除病原体并提供针对再次感染的长期免疫力。在初次感染和再次感染单核细胞增生李斯特菌期间，脾脏中CD4 +和CD8 + T细胞以及自然杀伤细胞的数量会增加，然而，CD8 + T细胞的比例增加最大。在免疫脾细胞中，表达低水平CD62L（一种激活标志）的CD8 T细胞比例增加，这表明单核细胞增生李斯特菌特异性CTL大量扩增。对免疫显性LLO 91-99表位特异性CTL的分析表明，在初次反应期间，基本上所有CTL都是CD62Llo，但在记忆阶段，许多CTL上调了CD62L的表达。有趣的是，在再次感染单核细胞增生李斯特菌期间，在脾脏中检测到的几乎所有额外的CD62Llo CTL的抗原特异性都可以用与四种不同表位复合的MHC I类四聚体来解释。这些研究证明了细胞内病原体感染期间复杂的T淋巴细胞动态变化。
