McDonald R J, Usachencko J
Department of Pediatrics and California Regional Primate Research Center, University of California, Davis, Sacramento 95817, USA.
Inflammation. 1999 Feb;23(1):63-73. doi: 10.1023/a:1020291617820.
Neutrophil (PMN) influx is an early, prominent finding in the airways of humans after experimental inhalation of ozone (O3), however the potential for PMN to contribute to epithelial injury in this setting is unknown. Bronchial epithelial cells of the human BEAS 2B R1.4 cell line or primary human bronchial epithelial cells underwent DNA labeling by incubation with BrdU. Monolayers were exposed to O3 (0.05 to 1 ppm) or filtered air for 60 min., and subsequently incubated with PMN for 2 h. Epithelial cell cytolysis was significant only in BEAS exposed to O3 and co-cultured with PMN. Apoptosis was maximal in BEAS exposed to O3 + PMN. Primary bronchial epithelial cells were resistant to injury; no cytolysis was detected, and apoptosis was detected only after treatment with 10 mM H2O2. Neutrophils may increase damage to the respiratory epithelium after O3 exposure, but primary bronchial epithelial cells are resistant to PMN and ozone induced injury.
在实验性吸入臭氧（O₃）后，中性粒细胞（PMN）流入是人类气道中早期出现的一个显著现象，然而在这种情况下PMN导致上皮损伤的可能性尚不清楚。人BEAS 2B R1.4细胞系的支气管上皮细胞或原代人支气管上皮细胞通过与溴脱氧尿苷（BrdU）孵育进行DNA标记。将单层细胞暴露于O₃（0.05至1 ppm）或过滤空气中60分钟，随后与PMN孵育2小时。上皮细胞溶解仅在暴露于O₃并与PMN共培养的BEAS细胞中显著。凋亡在暴露于O₃ + PMN的BEAS细胞中最大。原代支气管上皮细胞对损伤具有抗性；未检测到细胞溶解，仅在用10 mM过氧化氢处理后才检测到凋亡。中性粒细胞可能会增加O₃暴露后对呼吸道上皮的损伤，但原代支气管上皮细胞对PMN和臭氧诱导的损伤具有抗性。
