Li J, Fritz J S
Department of Chemistry and Ames Laboratory, Iowa State University, 50011, USA.
Electrophoresis. 1999 Jan;20(1):84-91. doi: 10.1002/(SICI)1522-2683(19990101)20:1<84::AID-ELPS84>3.0.CO;2-V.
For the separation of neutral compounds by micellar electrokinetic chromatography, separations are usually carried out in predominantly aqueous solution in order to preserve the charged micelle necessary for the separation. We now show that polycyclic aromatic hydrocarbon (PAH) compounds can be separated efficiently by capillary electrophoresis in pure methanol or in aqueous-organic mixtures containing a high percentage of methanol. Sodium tetradecyl sulfate was the preferred surfactant. The effects of pH, solvent composition, surfactant structure, and surfactant concentration on the separations were studied. Reproducible migration times and linear calibration plots were obtained.
对于通过胶束电动色谱法分离中性化合物，分离通常在主要为水溶液中进行，以保留分离所需的带电胶束。我们现在表明，多环芳烃（PAH）化合物可通过毛细管电泳在纯甲醇或含有高百分比甲醇的水 - 有机混合物中有效分离。十四烷基硫酸钠是优选的表面活性剂。研究了pH、溶剂组成、表面活性剂结构和表面活性剂浓度对分离的影响。获得了可重现的迁移时间和线性校准曲线。
