Akbay C, Shamsi S A, Warner I M
Department of Chemistry, Louisiana State University, Baton Rouge, LA, USA.
Electrophoresis. 1997 Feb;18(2):253-9. doi: 10.1002/elps.1150180213.
A double alkyl chain di(2-ethylhexyl)phosphate (DEHP) is introduced as a potential anionic micellar pseudophase for a wide range of benzene derivatives and/or polycyclic aromatic hydrocarbons (PAHs). Several parameters such as concentration of phosphated surfactant, type and concentration of organic solvents (acetonitrile, isopropanol, and methanol), as well as capillary electrophoresis separation voltage were optimized to enhance resolution, efficiency and selectivity as well as to maximize peak capacities. The migration times and selectivity order for a number of PAHs differ significantly, depending on the type of organic solvent added to the DEHP surfactant. Acetonitrile at a concentration of 30% v/v in combination with 100 mM DEHP gave optimum separation for a mixture of 21 benzene derivatives and PAHs in under 16 min.
引入双烷基链磷酸二（2-乙基己基）酯（DEHP）作为适用于多种苯衍生物和/或多环芳烃（PAH）的潜在阴离子胶束假相。对诸如磷酸化表面活性剂浓度、有机溶剂（乙腈、异丙醇和甲醇）的类型和浓度以及毛细管电泳分离电压等多个参数进行了优化，以提高分辨率、效率和选择性，并使峰容量最大化。多种PAH的迁移时间和选择性顺序差异显著，这取决于添加到DEHP表面活性剂中的有机溶剂类型。在100 mM DEHP中加入浓度为30% v/v的乙腈，可在16分钟内对21种苯衍生物和PAH的混合物实现最佳分离。
