Allen D J, Wall W E, Denson K D, Smith J T
Department of Physical Sciences, Southeastern Oklahoma State University, Durant 74701, USA.
Electrophoresis. 1999 Jan;20(1):100-10. doi: 10.1002/(SICI)1522-2683(19990101)20:1<100::AID-ELPS100>3.0.CO;2-1.
In this report, we introduce a new micelle modifier useful to alter selectivity in micellar electrokinetic capillary chromatography (MECC). 1,2-Hexanediol acts as a class I organic modifier in that its effects are on the sodium dodecyl sulfate (SDS) micellar rather than the surrounding aqueous phase. This characteristic allows 1,2-hexanediol to improve resolution when applied at concentrations as low as 20 mM (0.25% v/v) by altering the selectivity observed with SDS alone. The effects of 1,2-hexanediol on the critical micelle concentration of SDS, electroosmotic flow, electrophoretic mobility of the SDS micelle, and reproducibility are presented. 1,2-Hexanediol had little impact on the migration time window at concentrations below 100 mM. Changes in selectivity induced by 1,2-hexanediol for a large set of model compounds are presented. Analytes capable of forming hydrogen bonds tend to decrease their interactions with the micellar phase while nonhydrogen bonding analytes increase their interactions. The usefulness of 1,2-hexanediol was demonstrated by examining its effects on the separation of dansylated amino acids. Eighteen of twenty amino acids could be separated with a resolution greater than 1.6 within 1600 s using a combination of 1,2-hexanediol and isopropanol.
在本报告中，我们介绍了一种新型胶束改性剂，它可用于改变胶束电动毛细管色谱法（MECC）中的选择性。1,2 -己二醇作为I类有机改性剂，其作用于十二烷基硫酸钠（SDS）胶束而非周围的水相。这一特性使得1,2 -己二醇在浓度低至20 mM（0.25% v/v）时应用，通过改变单独使用SDS时观察到的选择性来提高分离度。本文介绍了1,2 -己二醇对SDS临界胶束浓度、电渗流、SDS胶束的电泳迁移率以及重现性的影响。在浓度低于100 mM时，1,2 -己二醇对迁移时间窗口影响很小。本文还介绍了1,2 -己二醇对大量模型化合物诱导的选择性变化。能够形成氢键的分析物往往会减少其与胶束相的相互作用，而非氢键结合的分析物则会增加其相互作用。通过研究1,2 -己二醇对丹磺酰化氨基酸分离的影响，证明了其有效性。使用1,2 -己二醇和异丙醇的组合，在1600 s内，20种氨基酸中的18种能够以大于1.6的分离度进行分离。
