使用聚合阴离子表面活性剂对十二种单甲基苯并[a]蒽异构体进行电动色谱分析。
Electrokinetic chromatography of twelve monomethylbenz[a]anthracene isomers using a polymerized anionic surfactant.
作者信息
Akbay C, Warner I M, Shamsi S A
机构信息
Department of Chemistry, Louisiana State University, Baton Rouge 70803, USA.
出版信息
Electrophoresis. 1999 Jan;20(1):145-51. doi: 10.1002/(SICI)1522-2683(19990101)20:1<145::AID-ELPS145>3.0.CO;2-2.
A method for the separation of twelve monomethyl-substituted benz[a]anthracene isomers using poly-(sodium undecylenic sulfate) (poly-SUS) surfactant by means of electrokinetic capillary chromatography (EKC) is described. Several parameters such as concentration of acetonitrile (ACN), pH, as well as applied voltage were studied to optimize the EKC separation. ACN at a concentration of 35% v/v, 12.5 mM phosphate-borate buffer, 30 kV with 0.5% w/v poly-SUS at a pH of 9.5 provided a resolution of a mixture of nine out of twelve methylbenz[a]anthracene (MBA) isomers in 50 min. The results of this study suggest that molecular length of MBA rather than length-to-breath ratio plays an important role in the elution order of some isomers.
描述了一种使用聚（十一烷基硫酸钠）（聚-SUS）表面活性剂通过电动毛细管色谱法（EKC）分离十二种单甲基取代苯并[a]蒽异构体的方法。研究了几个参数，如乙腈（ACN）浓度、pH值以及施加电压，以优化EKC分离。在pH为9.5时，35% v/v的ACN、12.5 mM磷酸盐-硼酸盐缓冲液、30 kV以及0.5% w/v的聚-SUS，可在50分钟内分离出十二种甲基苯并[a]蒽（MBA）异构体中的九种混合物。这项研究结果表明，MBA的分子长度而非长宽比在某些异构体的洗脱顺序中起重要作用。
Zotero 插件