Maher M P, Pine J, Wright J, Tai Y C
Division of Biology, California Institute of Technology, Pasadena 91125, USA.
J Neurosci Methods. 1999 Feb 1;87(1):45-56. doi: 10.1016/s0165-0270(98)00156-3.
The neurochip is a silicon micromachined device upon which cultured mammalian neurons can be continuously and individually monitored and stimulated. The neurochip is based upon a 4 x 4 array of metal electrodes, each of which has a caged well structure designed to hold a single mature cell body while permitting normal outgrowth of neural processes. We demonstrate that this device is capable of maintaining cell survival, and that the electrodes can both record and stimulate electrical activity in individual cells with no crosstalk between channels.
神经芯片是一种硅微加工设备，在其上可以对培养的哺乳动物神经元进行连续且单独的监测和刺激。该神经芯片基于一个4×4的金属电极阵列，每个电极都有一个笼状阱结构，旨在容纳单个成熟的细胞体，同时允许神经突起正常生长。我们证明了该设备能够维持细胞存活，并且电极能够记录和刺激单个细胞中的电活动，通道之间没有串扰。
