Hart A C, Kass J, Shapiro J E, Kaplan J M
Department of Pathology, Harvard Medical School, Boston, Massachusetts 02114, USA.
J Neurosci. 1999 Mar 15;19(6):1952-8. doi: 10.1523/JNEUROSCI.19-06-01952.1999.
The Caenorhabditis elegans ASH sensory neurons mediate responses to nose touch, hyperosmolarity, and volatile repellent chemicals. We show here that distinct signaling pathways mediate the responses to touch and hyperosmolarity. ASH neurons distinguish between these stimuli because habituation to nose touch has no effect on the response to high osmolarity or volatile chemicals (1-octanol). Mutations in osm-10 eliminate the response to hyperosmolarity but have no effect on responses to nose touch or to volatile repellents. OSM-10 is a novel cytosolic protein expressed in ASH and three other classes of sensory neurons. Mutations in two other osmosensory-defective genes, eos-1 and eos-2, interact genetically with osm-10. Our analysis suggests that nose touch sensitivity and osmosensation occur via distinct signaling pathways in ASH and that OSM-10 is required for osmosensory signaling.
秀丽隐杆线虫的ASH感觉神经元介导对鼻尖触碰、高渗以及挥发性驱避化学物质的反应。我们在此表明，不同的信号通路介导对触碰和高渗的反应。ASH神经元能够区分这些刺激，因为对鼻尖触碰的习惯化对高渗或挥发性化学物质（1-辛醇）的反应没有影响。osm-10基因突变消除了对高渗的反应，但对鼻尖触碰或挥发性驱避剂的反应没有影响。OSM-10是一种在ASH以及其他三类感觉神经元中表达的新型胞质蛋白。另外两个渗透压感觉缺陷基因eos-1和eos-2的突变与osm-10发生遗传相互作用。我们的分析表明，鼻尖触碰敏感性和渗透压感觉通过ASH中不同的信号通路发生，并且OSM-10是渗透压感觉信号传导所必需的。
