Yoshino S, Sagai M
Department of Microbiology, Saga Medical School, Saga, 849-8501, Japan.
Cell Immunol. 1999 Feb 25;192(1):72-8. doi: 10.1006/cimm.1998.1427.
The present study was undertaken to examine whether oral administration of soluble antigen together with diesel exhaust particles (DEP) induced the systemic immune response in mice. Mice were orally given 1 mg of hen egg lysozyme (HEL) with varying doses of DEP every 3 days over a period of 15 days. The results showed that oral administration of HEL plus DEP produced anti-HEL IgG antibodies in serum in a dose-related fashion, while either HEL or DEP alone failed to show the antigen-specific IgG antibody production. Production of anti-HEL IgG2a and IgG1 antibodies, which are dependent on Th1 and Th2 CD4(+) T cells, respectively, was seen in mice fed with combined HEL and DEP, although anti-HEL IgG1 antibodies appeared to be more efficiently produced by lower doses of DEP than anti-HEL IgG2a antibodies. There was marked antigen-specific proliferation of spleen cells in mice treated with HEL and DEP. The anti-HEL antibody production and lymphoid cell proliferation to the antigen were associated with marked secretion of the Th1 cytokine IFN-gamma as well as the Th2 cytokine IL-4. These results suggest that DEP may act as a mucosal adjuvant in the gut enhancing systemic Th1 and Th2 immune responses and might play a role in oral immunization and food allergy.
本研究旨在探讨口服可溶性抗原与柴油废气颗粒（DEP）共同给药是否能诱导小鼠产生全身免疫反应。在15天的时间里，每隔3天给小鼠口服1毫克鸡蛋溶菌酶（HEL）并搭配不同剂量的DEP。结果显示，口服HEL加DEP能以剂量相关的方式在血清中产生抗HEL IgG抗体，而单独给予HEL或DEP均未显示出抗原特异性IgG抗体的产生。在同时喂食HEL和DEP的小鼠中，分别依赖Th1和Th2 CD4(+) T细胞产生了抗HEL IgG2a和IgG1抗体，尽管较低剂量的DEP似乎比抗HEL IgG2a抗体更有效地产生抗HEL IgG1抗体。用HEL和DEP处理的小鼠脾脏细胞出现明显的抗原特异性增殖。抗HEL抗体的产生以及淋巴细胞对抗原的增殖与Th1细胞因子IFN-γ以及Th2细胞因子IL-4的显著分泌有关。这些结果表明，DEP可能作为肠道中的黏膜佐剂，增强全身Th1和Th2免疫反应，并可能在口服免疫和食物过敏中发挥作用。
