Kunz M, Henseleit-Walter U, Sorg C, Kolde G
Department of Dermatology, University of Würzburg, 97080 Würzburg, Germany.
Eur J Dermatol. 1999 Mar;9(2):107-10.
Chronic inflammatory discoid lupus erythematosus (DLE) and Jessner's lymphocytic infiltration of the skin (LIS) are both characterized by dermal infiltrates of activated T lymphocytes. However, an inflammatory involvement of the epidermis is only found in DLE. We therefore compared the phenotypic properties of the keratinocytes using immunohistochemical stainings of biopsies from typical DLE and LIS. Keratinocytes failed to express HLA-DR in LIS and surprisingly also in DLE. The adhesion molecule ICAM-1 was only expressed in DLE, with focal staining of the basal keratinocytes in close association with intraepidermal lymphocytes. The monoclonal antibody 27E10, a distinct marker for macrophage activation and differentiation, revealed a strong band-like labelling of the suprabasal and upper keratinocytes in DLE. In contrast, no epidermal expression of this biologically active heterodimer of the calcium-binding proteins MRP-8 and MRP-14 was found in LIS. The staining patterns provide a new method to differentiate DLE and LIS by immunohistochemistry and suggest a distinct type of keratinocyte activation and differentiation in DLE which would in turn mediate epidermal T cell infiltration.
慢性炎症性盘状红斑狼疮（DLE）和杰斯纳皮肤淋巴细胞浸润症（LIS）均以活化T淋巴细胞的真皮浸润为特征。然而，表皮的炎症累及仅见于DLE。因此，我们使用典型DLE和LIS活检组织的免疫组织化学染色，比较了角质形成细胞的表型特性。角质形成细胞在LIS中未能表达HLA-DR，令人惊讶的是在DLE中也未表达。黏附分子ICAM-1仅在DLE中表达，在基底角质形成细胞有局灶性染色，与表皮内淋巴细胞密切相关。单克隆抗体27E10是巨噬细胞活化和分化的独特标志物，在DLE中显示基底上层和上层角质形成细胞有强烈的带状标记。相比之下，在LIS中未发现钙结合蛋白MRP-8和MRP-14这种生物活性异二聚体的表皮表达。这些染色模式提供了一种通过免疫组织化学区分DLE和LIS的新方法，并提示DLE中存在一种独特类型的角质形成细胞活化和分化，这反过来又会介导表皮T细胞浸润。
