Jeong K I, Uetsuka K, Nakayama H, Doi K
Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Japan.
Exp Anim. 1999 Jan;48(1):23-9. doi: 10.1538/expanim.48.23.
We examined lectin-histochemically the glycoconjugate expression in the follicle-associated epithelium (FAE) covering the nasal-associated lymphoid tissue (NALT) in the rat under specific pathogen-free (SPF) and conventional (CV) conditions and compared the results for SPF and CV rats as well as for membranous (M) cells and adjacent ciliated respiratory epithelial (CRE) cells in FAE. N-acetylgalactosamine-specific lectins, Dolichos biflorus (DBA), Helix pomatia (HPA), Glycine max (SBA) and Vicia villosa (VVA), and alpha-L-fucose-specific lectin, Ulex europaeus (UEA-I), preferentially bound to M cells mainly in the luminal surface compared with CRE cells in SPF rats, whereas DBA and UEA-I showed signs of preferential binding to the apical and basolateral cytoplasm as well as to the luminal surface of M cells in CV rats. In addition, HPA, SBA and VVA more frequently and extensively labeled M cells than CRE cells in CV rats with the same subcellular staining pattern as DBA and UEA-I. On the whole, the changes in lectin binding frequency and strength were more prominent in M cells than in CRE cells in both SPF and CV rats. The present results indicate that DBA and UEA-I are useful as markers of M cells in NALT. Furthermore, the pattern of expression of carbohydrate residues recognized by such lectins in SPF and CV rats suggests that M cells are highly sensitive to environmental changes.
我们采用凝集素组织化学方法，研究了在无特定病原体（SPF）和常规（CV）条件下，大鼠鼻相关淋巴组织（NALT）上覆盖的滤泡相关上皮（FAE）中的糖缀合物表达情况，并比较了SPF大鼠和CV大鼠以及FAE中膜性（M）细胞和相邻纤毛呼吸上皮（CRE）细胞的结果。N-乙酰半乳糖胺特异性凝集素，双花扁豆（DBA）、苹果蜗牛（HPA）、大豆（SBA）和绒毛野豌豆（VVA），以及α-L-岩藻糖特异性凝集素，欧洲荆豆（UEA-I），与SPF大鼠的CRE细胞相比，主要在管腔表面优先结合M细胞，而在CV大鼠中，DBA和UEA-I显示出优先结合M细胞的顶端和基底外侧细胞质以及管腔表面的迹象。此外，在CV大鼠中，HPA、SBA和VVA比CRE细胞更频繁、更广泛地标记M细胞，其亚细胞染色模式与DBA和UEA-I相同。总体而言，在SPF和CV大鼠中，凝集素结合频率和强度的变化在M细胞中比在CRE细胞中更明显。目前的结果表明，DBA和UEA-I可作为NALT中M细胞的标记物。此外，此类凝集素在SPF和CV大鼠中识别的碳水化合物残基的表达模式表明，M细胞对环境变化高度敏感。
