Takata S, Ohtani O, Watanabe Y
Department of Anatomy, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Sugitani, Japan.
Arch Histol Cytol. 2000 Oct;63(4):305-12. doi: 10.1679/aohc.63.305.
We investigated the binding of four types of lectin to follicle-associated epithelium overlying the nasal-associated lymphoid tissue (NALT) of rats in order to identify M-cell specific surface markers and to determine the influence of lectin administration to NALT on the uptake of a particulate antigen. The NALT tissues were incubated with a panel of four types of lectin conjugated to horseradish peroxidase (HRP). Ulex europaeus-1 (UEA-1) and Dolichos biflorus (DBA) lectin stained the surface of M-cells and goblet cells. Uniform staining by Triticum vulgaris (WGA) was detected in the M-cells, ciliated cells and goblet cells. In contrast, staining of Griffonia simplicifolia I isolectin-B4 (GSI-B4) was almost exclusively M-cell specific. The administration of M-cell specific lectin (GS I-B4) to NALT suppressed the uptake of baker's yeast particles administered later, whereas the non-specific one (UEA-1) had no influence on the uptake. These results indicate that GS I-B4 is a useful marker for the identification of rat NALT M-cells and that such a specific expression of surface glycoconjugates by M-cells may permit the targeting of vaccines and drugs to the antigen sampling sites of the nose. It also appears possible to block the uptake of pathogens by an administration of M-cell specific lectin to NALT.
我们研究了四种凝集素与大鼠鼻相关淋巴组织（NALT）上方的滤泡相关上皮细胞的结合情况，以鉴定M细胞特异性表面标志物，并确定向NALT施用凝集素对颗粒性抗原摄取的影响。将NALT组织与一组与辣根过氧化物酶（HRP）偶联的四种凝集素一起孵育。荆豆凝集素-1（UEA-1）和双花扁豆凝集素（DBA）对M细胞和杯状细胞的表面进行了染色。在M细胞、纤毛细胞和杯状细胞中均检测到普通小麦凝集素（WGA）的均匀染色。相比之下，西非单叶豆凝集素I同工凝集素-B4（GSI-B4）的染色几乎完全是M细胞特异性的。向NALT施用M细胞特异性凝集素（GS I-B4）可抑制随后施用的面包酵母颗粒的摄取，而非特异性凝集素（UEA-1）对摄取没有影响。这些结果表明，GS I-B4是鉴定大鼠NALT M细胞的有用标志物，并且M细胞表面糖缀合物的这种特异性表达可能使疫苗和药物能够靶向鼻子的抗原采样部位。通过向NALT施用M细胞特异性凝集素，似乎也有可能阻断病原体的摄取。
