用轮状病毒对小鼠进行序贯口服-胃肠外免疫刺激产生的抗体反应和保护作用。

Antibody responses and protection stimulated by sequential oral-parenteral immunization of mice with rotavirus.

作者信息

McNeal M M, Rae M N, Ward R L

机构信息

Division of Infectious Diseases, Children's Hospital Medical Center, Cincinnati, OH 45229, USA.

出版信息

Vaccine. 1999 Feb 26;17(7-8):639-45. doi: 10.1016/s0264-410x(98)00244-8.

DOI:10.1016/s0264-410x(98)00244-8

PMID:10067668

Abstract

Antibody responses and protection against shedding following oral challenge with murine rotavirus (EDIM) were determined in mice after sequential oral parenteral immunization. Oral immunization of 4-day-old BALB/c mice with live, heterologous rotavirus (RRV) stimulated serum rotavirus IgG but little serum or intestinal rotavirus IgA and small but significant (P < 0.001) reductions in EDIM shedding. Intraperitoneal immunization with inactivated EDIM at 29 days of age had similar effects. Sequential oral-parenteral immunization under these conditions stimulated small but significant (P < 0.001) increases in both rotavirus IgG and IgA titers and reduced shedding (P < 0.001) compared to individual immunizations. However, these responses were essentially additive, indicative of separate inductive/effector sites for mucosal and systemic immunity.

摘要

在小鼠经序贯口服-肠外免疫后，测定了其对鼠轮状病毒（EDIM）口服攻击后的抗体反应及防止病毒排出的能力。用活的异源轮状病毒（RRV）对4日龄BALB/c小鼠进行口服免疫，可刺激血清轮状病毒IgG产生，但血清或肠道轮状病毒IgA产生较少，且EDIM排出量有小幅度但显著（P<0.001）的减少。29日龄时用灭活的EDIM进行腹腔免疫也有类似效果。在这些条件下，序贯口服-肠外免疫与单独免疫相比，可刺激轮状病毒IgG和IgA滴度有小幅度但显著（P<0.001）的增加，并减少病毒排出（P<0.001）。然而，这些反应基本上是相加的，表明黏膜免疫和全身免疫有各自独立的诱导/效应位点。