Steger K K, Valentine P J, Heffron F, So M, Pauza C D
Department of Pathology and Laboratory Medicine, University of Wisconsin Medical School, Madison, USA.
Vaccine. 1999 Feb 26;17(7-8):923-32. doi: 10.1016/s0264-410x(98)00278-3.
Recombinant bacteria are useful vectors for delivering foreign antigens to mucosal surfaces and may elicit immune protection against sexually-transmitted pathogens. Recombinant, attenuated Salmonella typhimurium expressing the Simian Immunodeficiency Virus capsid protein (p27) were given to rhesus macaques by intragastric intubation. This route of immunization was compared with intramuscular injection of soluble p27 in adjuvant, and with immunization protocols that combined intragastric and intramuscular antigen exposures. Recombinant Salmonella stimulated p27-specific lymphoproliferative responses that were present transiently in peripheral blood, and were recalled easily by booster immunizations. Intramuscular p27 injection elicited strong serum antibody responses, but only low level capsid-specific proliferative responses. Recombinant Salmonella immunization elicited low levels of p27-specific antibodies in serum and did not suppress subsequent responses to parenteral immunization. Intragastric immunization of macaques with recombinant Salmonella typhimurium was safe and induced immune responses specific for the expressed, foreign antigen.
重组细菌是将外源抗原递送至黏膜表面的有用载体，并且可能引发针对性传播病原体的免疫保护。通过胃内插管将表达猿猴免疫缺陷病毒衣壳蛋白（p27）的重组减毒鼠伤寒沙门氏菌给予恒河猴。将这种免疫途径与在佐剂中肌肉注射可溶性p27以及将胃内和肌肉内抗原暴露相结合的免疫方案进行比较。重组沙门氏菌刺激了p27特异性淋巴细胞增殖反应，该反应短暂存在于外周血中，并且通过加强免疫很容易被再次激发。肌肉注射p27引发了强烈的血清抗体反应，但仅引发了低水平的衣壳特异性增殖反应。重组沙门氏菌免疫在血清中引发了低水平的p27特异性抗体，并且没有抑制随后对肠外免疫的反应。用重组鼠伤寒沙门氏菌对猕猴进行胃内免疫是安全的，并诱导了针对所表达的外源抗原的特异性免疫反应。
