Burgos J M, Perez J L, Garcia L, Gonzalez G S, Benitez J A, Galindo F, Silberstein C, Ibarra C
Departamento de Fisiología, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
Vaccine. 1999 Feb 26;17(7-8):949-56. doi: 10.1016/s0264-410x(98)00310-7.
The recent spread of El Tor cholera in Latin America highlights the need for a safe and economical vaccine. The main approach for developing live recombinant vaccines has been to disarm known pathogenic strains of cholera toxin leaving intact antigens involved in protection. These recombinant vaccine candidates do not cause severe diarrhea, but they are too reactogenic for wide scale usage. We describe here a test capable of determining the diarrheagenic potential of attenuated V. cholerae strains. The functional test consists in the simultaneous recording of net water movement, electrical potential difference and short-circuit current across the human intestine ex vivo. We found that human tissues incubated with supernatants from the attenuated 638, 413 and 251a V. cholerae strains caused no changes in the ion conductances and water absorption in ileal and colon tissues allowing them to be assayed in volunteers.
近期埃尔托霍乱在拉丁美洲的传播凸显了对一种安全且经济的疫苗的需求。开发活重组疫苗的主要方法是使已知的霍乱毒素致病菌株减毒，同时保留参与保护作用的抗原。这些重组候选疫苗不会引起严重腹泻，但它们的反应原性过高，无法大规模使用。我们在此描述一种能够确定减毒霍乱弧菌菌株致腹泻潜力的测试方法。该功能测试包括在离体条件下同时记录穿过人体肠道的净水分移动、电势差和短路电流。我们发现，用减毒的638、413和251a霍乱弧菌菌株的上清液孵育的人体组织，在回肠和结肠组织中不会引起离子电导和水分吸收的变化，从而可以在志愿者身上进行检测。
