Gan L, Fagerholm P, Kim H J
St. Erik's Eye Hospital, Karolinska Institute, Stockholm, Sweden.
Invest Ophthalmol Vis Sci. 1999 Mar;40(3):575-81.
To establish whether fucoidin, by blocking the adhesion of leukocytes on the limbal vascular endothelium, prevents extravasation of the cells from the blood stream into the limbal stroma and the wounded area after corneal injury. Successful leukocyte blocking enabled investigation of the influence of leukocytes on corneal cellular proliferation after corneal wounding.
Thirty-two New Zealand White rabbits were used. Photorefractive keratectomy (PRK) and a standardized alkali corneal wound were used as models in two sets of experiments. In half of the injured rabbits fucoidin was used to prevent leukocytes from leaving the local vessels. The efficiency of the blocking technique was evaluated by counting the number of leukocytes in the limbal and wounded corneal areas. Proliferating cell nuclear antigen (PCNA) was used as a marker for proliferative activity.
The infiltration of leukocytes into the limbus and the cornea after PRK and alkali injuries can be blocked by fucoidin. The healing rate of corneal epithelium after alkali burn was retarded in the absence of leukocytes. PCNA expression was enhanced in the presence of leukocytes. Fucoidin per se had no influence on corneal cell proliferation and wound healing.
Polymorphonuclear leukocytes (PMNs) can be prevented from entering the cornea in vivo by fucoidin after PRK and after alkali burn. The corneal epithelial healing rate is delayed in the absence of PMNs in vivo, and PCNA expression increases in the presence of leukocytes.
通过阻断岩藻依聚糖对角膜缘血管内皮细胞上白细胞的黏附作用，确定其是否能防止角膜损伤后白细胞从血流中渗出到角膜缘基质和损伤区域。成功阻断白细胞后，可研究白细胞对角膜损伤后细胞增殖的影响。
使用32只新西兰白兔。在两组实验中，分别采用准分子激光原位角膜磨镶术（PRK）和标准化碱性角膜伤口作为模型。在一半受伤的兔子中，使用岩藻依聚糖来防止白细胞离开局部血管。通过计数角膜缘和受伤角膜区域的白细胞数量来评估阻断技术的效果。增殖细胞核抗原（PCNA）用作增殖活性的标志物。
PRK和碱性损伤后，岩藻依聚糖可阻断白细胞向角膜缘和角膜的浸润。在没有白细胞的情况下，碱烧伤后角膜上皮的愈合速度减慢。白细胞存在时PCNA表达增强。岩藻依聚糖本身对角膜细胞增殖和伤口愈合没有影响。
PRK和碱烧伤后，岩藻依聚糖可在体内防止多形核白细胞（PMN）进入角膜。体内没有PMN时，角膜上皮愈合速度延迟，白细胞存在时PCNA表达增加。
