慢性活动性EB病毒感染患者外周血淋巴细胞中EB病毒（EBV）载量持续居高不下。

Persistently high Epstein-Barr virus (EBV) loads in peripheral blood lymphocytes from patients with chronic active EBV infection.

作者信息

Maeda A, Wakiguchi H, Yokoyama W, Hisakawa H, Tomoda T, Kurashige T

机构信息

Microbiology and Tumorbiology Center (MTC), Karolinska Institute, S-171 77, Stockholm, Sweden.

出版信息

J Infect Dis. 1999 Apr;179(4):1012-5. doi: 10.1086/314691.

DOI:10.1086/314691

PMID:10068600

Abstract

Chronic active Epstein-Barr virus infection (CAEBV) is a severe illness with unusual EBV activation that persists for years, and its pathogenesis is largely unknown. After the creation of an accurate and reproducible polymerase chain reaction system to quantify EBV DNA, virus loads in peripheral blood lymphocytes (PBL) were determined in 54 children: 15 with CAEBV, 16 with infectious mononucleosis (IM), and 23 healthy children. Children with CAEBV and those with IM had high virus loads. Lower loads were detected in 47% of seropositive healthy donors. There were two distinct differences between children with CAEBV and those with IM: The former had greater viral replication (10(3)-10(7) copies/2.5x10(5) PBL) than those with IM, and viral replication declined in children with IM whereas active replication persisted for years in subjects with CAEBV. Persisting high virus loads are a possible diagnostic criterion for CAEBV. EBV loads may enable classification and prognosis of EBV infections.

摘要

慢性活动性EB病毒感染（CAEBV）是一种严重疾病，伴有异常的EB病毒激活，持续数年，其发病机制在很大程度上尚不清楚。在创建了准确且可重复的聚合酶链反应系统以定量EB病毒DNA后，测定了54名儿童外周血淋巴细胞（PBL）中的病毒载量：15名患有CAEBV，16名患有传染性单核细胞增多症（IM），23名健康儿童。患有CAEBV的儿童和患有IM的儿童病毒载量较高。在47%的血清阳性健康供者中检测到较低的病毒载量。患有CAEBV的儿童和患有IM的儿童之间存在两个明显差异：前者的病毒复制（10³-10⁷拷贝/2.5×10⁵个PBL）比患有IM的儿童更多，并且患有IM的儿童病毒复制下降，而患有CAEBV的受试者中活跃复制持续数年。持续的高病毒载量可能是CAEBV的一个诊断标准。EB病毒载量可能有助于EB病毒感染的分类和预后判断。