Miao H, Han K, Gu F
Institute of Pediatrics, Beijing Medical University, People's Republic of China.
Anal Quant Cytol Histol. 1999 Feb;21(1):89-92.
To quantitatively evaluate the changes in glomeruli in unilaterally nephrectomized adult rats by unbiased stereologic techniques.
Adult rats received unilateral nephrectomy (UN) or a sham operation. Four weeks later the remnant kidneys were fixed and sampled by the fractionator method. Consecutive sections were prepared and stained. Morphometric analyses were performed by a light microscope equipped with computer-assisted digitizer.
The total glomerular volume increased by 51% (P < .01). The volume density of glomeruli did not change (5.58 +/- 0.22 x 10(-2) vs. 5.51 +/- 0.44 x 10(-2), P > .50). In contrast, the numerical densities of glomeruli decreased by 36% (P < .05).
Glomerular enlargement, but not the increase in glomerular number contributed heavily to compensatory glomerular growth after UN in rats.
采用无偏倚立体学技术定量评估单侧肾切除成年大鼠肾小球的变化。
成年大鼠接受单侧肾切除术（UN）或假手术。四周后，将残余肾脏固定并采用分样法取样。制备连续切片并染色。通过配备计算机辅助数字化仪的光学显微镜进行形态计量分析。
肾小球总体积增加了51%（P < 0.01）。肾小球的体积密度未发生变化（5.58 ± 0.22×10⁻² 与 5.51 ± 0.44×10⁻²，P > 0.50）。相比之下，肾小球的数量密度下降了36%（P < 0.05）。
肾小球增大而非肾小球数量增加对大鼠单侧肾切除术后的代偿性肾小球生长起主要作用。
