利用T7表达系统过表达大肠杆菌的FNR蛋白。
Overexpression of the FNR protein of Escherichia coli with T7 expression system.
作者信息
Stuchlík S, Turna J
机构信息
Department of Molecular Biology, Comenius University, Bratislava, Slovakia.
出版信息
Folia Microbiol (Praha). 1998;43(6):601-4. doi: 10.1007/BF02816375.
PMID:10069008
Abstract
We have used the T7 expression system for expression of E. coli FNR protein. The fnr gene was cloned from its initiation codon ATG into the NdeI site of an expression vector and filamentous phage mGP1-2 was used as a donor of T7 RNA polymerase gene. The level of FNR expression attained by this expression arrangement was about 45% of total cell proteins.
摘要
我们使用T7表达系统来表达大肠杆菌FNR蛋白。将fnr基因从其起始密码子ATG克隆到表达载体的NdeI位点，并使用丝状噬菌体mGP1-2作为T7 RNA聚合酶基因的供体。通过这种表达安排所达到的FNR表达水平约占总细胞蛋白的45%。
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