表达Pto抗病基因的植物对含有无毒基因AvrPto的重组PVX具有抗性。

Plants expressing the Pto disease resistance gene confer resistance to recombinant PVX containing the avirulence gene AvrPto.

作者信息

Tobias C M, Oldroyd G E, Chang J H, Staskawicz B J

机构信息

Department of Plant and Microbial Biology, University of California, Berkeley 94720, USA.

出版信息

Plant J. 1999 Jan;17(1):41-50. doi: 10.1046/j.1365-313x.1999.00350.x.

DOI:10.1046/j.1365-313x.1999.00350.x

PMID:10069066

Abstract

Elicitation of hypersensitive cell death and induction of plant disease resistance by Pseudomonas syringae pv. tomato (Pst) is dependent on activity of the Pst Hrp secretion system and the gene-for-gene interaction between the tomato resistance gene Pto and the bacterial avirulence gene avrPto. AvrPto was expressed transiently in resistant or susceptible plant lines via a potato virus X (PVX) vector. We found that while PVX is normally virulent on tomato, a PVX derivative expressing avrPto was only capable of infecting plants lacking a functional Pto resistance pathway. Mutations in either the Pto or Prf genes allowed systemic spread of the recombinant virus. These results indicate that recognition of AvrPto by Pto in resistant plant lines triggers a plant defense response that can confer resistance to a viral as well as a bacterial pathogen.

摘要

丁香假单胞菌番茄致病变种（Pst）引发的过敏细胞死亡及植物抗病性诱导依赖于Pst Hrp分泌系统的活性以及番茄抗病基因Pto与细菌无毒基因avrPto之间的基因对基因相互作用。通过马铃薯X病毒（PVX）载体在抗性或感病植物品系中瞬时表达AvrPto。我们发现，虽然PVX通常对番茄具有毒性，但表达avrPto的PVX衍生物仅能感染缺乏功能性Pto抗性途径的植物。Pto或Prf基因中的突变允许重组病毒的系统传播。这些结果表明，抗性植物品系中Pto对AvrPto的识别引发了一种植物防御反应，该反应可赋予对病毒和细菌病原体的抗性。