Choi Sera, Jayaraman Jay, Segonzac Cécile, Park Hye-Jee, Park Hanbi, Han Sang-Wook, Sohn Kee Hoon
Bioprotection Research Centre, Institute of Agriculture and Environment, Massey University, Palmerston North, New Zealand.
Department of Life Sciences, Pohang University of Science and Technology, Pohang, South Korea.
Front Plant Sci. 2017 Dec 20;8:2157. doi: 10.3389/fpls.2017.02157. eCollection 2017.
Bacterial phytopathogen type III secreted (T3S) effectors have been strongly implicated in altering the interaction of pathogens with host plants. Therefore, it is useful to characterize the whole effector repertoire of a pathogen to understand the interplay of effectors in plants. pv. is a causal agent of kiwifruit canker disease. In this study, we generated an -mediated transient expression library of YFP-tagged T3S effectors from two strains of , -NZ V13 and -NZ LV5, in order to gain insight into their mode of action in and . Determining the subcellular localization of effectors gives an indication of the possible host targets of effectors. A confocal microscopy assay detecting YFP-tagged effectors revealed that the nucleus, cytoplasm and cell periphery are major targets of effectors. -mediated transient expression of multiple effectors induced HR-like cell death (HCD) in spp., suggesting that multiple effectors may be recognized by spp.. Virus-induced gene silencing (VIGS) of several known plant immune regulators, , , or specified the requirement of SGT1 in HCD induced by several effectors in . In addition, the suppression activity of effectors on HCD-inducing proteins and PTI was assessed. effectors showed differential suppression activities on each HCD inducer or PTI. Taken together, our effector repertoire analysis highlights the great diversity of T3S effector functions .
细菌植物病原体III型分泌（T3S）效应子与改变病原体与宿主植物的相互作用密切相关。因此，表征病原体的整个效应子库对于理解效应子在植物中的相互作用是有用的。 pv. 是猕猴桃溃疡病的病原体。在本研究中，我们构建了一个由 - 介导的、来自 -NZ V13和 -NZ LV5两个菌株的YFP标记的T3S效应子的瞬时表达文库，以便深入了解它们在 和 中的作用模式。确定效应子的亚细胞定位可以指示效应子可能的宿主靶标。检测YFP标记的 效应子的共聚焦显微镜分析表明，细胞核、细胞质和细胞周边是 效应子的主要靶标。 - 介导的多个 效应子的瞬时表达在 spp.中诱导了类似过敏反应的细胞死亡（HCD），这表明多个 效应子可能被 spp.识别。几种已知的植物免疫调节因子 、 、 或 的病毒诱导基因沉默（VIGS）确定了SGT1在 中几种 效应子诱导的HCD中的必要性。此外，评估了 效应子对HCD诱导蛋白和PTI的抑制活性。 效应子对每种HCD诱导剂或PTI表现出不同的抑制活性。综上所述，我们的 效应子库分析突出了T3S效应子功能的巨大多样性。
