Matsumiya A, Yamaguchi M, Nakano H, Takeda M, Kumada K
Department of Surgery, Showa University Fujigaoka Hospital, Yokohama, Japan.
Life Sci. 1999;64(2):PL9-17. doi: 10.1016/s0024-3205(98)00546-3.
Effects of heparin-like glycosaminoglycans (h-GAGs) on neutrophil adhesion to endothelial cells (ECs) were investigated under physiological flow conditions using human umbilical vein ECs. Neutrophil adhesion to lipopolysaccharide-activated ECs was assayed under rotating conditions with addition of h-GAGs or monoclonal antibodies (mAbs) against selectins. Neutrophil adhesion to activated ECs under rotating conditions was suppressed completely by anti-E-selectin mAb and partially by anti-L-selectin mAb. Addition of dextran sulfate or heparin also significantly inhibited neutrophil adhesion to ECs under the same conditions. Moreover, effects of h-GAGs on neutrophil adhesion to recombinant E-selectin-coated plates were analyzed. Anti-E-selectin mAb and dextran sulfate, but not heparin, significantly inhibited neutrophil adhesion to E-selectin-coated plates. Results suggest that dextran sulfate inhibited E-selectin-mediated neutrophil adhesion to endotoxin-activated ECs under physiologic flow conditions.
