从堆肥中分离出的细菌对不饱和脂肪酸的转化

Conversion of unsaturated fatty acids by bacteria isolated from compost.

作者信息

Kaneshiro T, Kuo T M, Nakamura L K

机构信息

Oil Chemical Reseach, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, IL 61604, USA.

出版信息

Curr Microbiol. 1999 Apr;38(4):250-5. doi: 10.1007/pl00006796.

DOI:10.1007/pl00006796

PMID:10069863

Abstract

A compost mixture amended with soybean oil was enriched in microorganisms that transformed unsaturated fatty acids (UFAs). When oleic acid or 10-ketostearic acid was the selective fatty acid, Sphingobacterium thalpophilum (NRRL B-23206, NRRL B-23208, NRRL B-23209, NRRL B-23210, NRRL B-23211, NRRL B-23212), Acinetobacter spp. (NRRL B-23207, NRRL B-23213), and Enterobacter cloacae (NRRL B-23264, NRRL B-23265, NRRL B-23266) represented isolates that produced either hydroxystearic acid, ketostearic acid, or incomplete decarboxylations. When ricinoleic (12-hydroxy-9-octadecenoic) acid was the selective UFA, Enterobacter cloacae (NRRL B-23257, NRRL B-23267) and Escherichia sp. (NRRL B-23259) produced 12-C and 14-C homologous compounds, and Pseudomonas aeruginosa (NRRL B-23256, NRRL B-23260) converted ricinoleate to a trihydroxyoctadecenoate product. Also, various Enterobacter, Pseudomonas, and Serratia spp. appeared to decarboxylate linoleate substrate incompletely. These saprophytic, compost bacteria were aerobic or facultative anaerobic Gram-negative and decomposed UFAs through decarboxylation, hydroxylation, and hydroperoxidation mechanisms.

摘要

用大豆油改良的堆肥混合物富含能转化不饱和脂肪酸（UFA）的微生物。当油酸或10-酮硬脂酸为选择性脂肪酸时，嗜盐碱鞘氨醇杆菌（NRRL B - 23206、NRRL B - 23208、NRRL B - 23209、NRRL B - 23210、NRRL B - 23211、NRRL B - 23212）、不动杆菌属（NRRL B - 23207、NRRL B - 23213）以及阴沟肠杆菌（NRRL B - 23264、NRRL B - 23265、NRRL B - 23266）是产生羟基硬脂酸、酮硬脂酸或不完全脱羧产物的分离菌株。当蓖麻油酸（12-羟基-9-十八碳烯酸）为选择性不饱和脂肪酸时，阴沟肠杆菌（NRRL B - 23257、NRRL B - 23267）和埃希氏菌属（NRRL B - 23259）产生12碳和14碳的同源化合物，铜绿假单胞菌（NRRL B - 23256、NRRL B - 23260）将蓖麻油酸转化为三羟基十八碳烯酸产物。此外，各种肠杆菌属、假单胞菌属和沙雷氏菌属似乎不完全脱羧亚油酸底物。这些腐生的堆肥细菌为需氧或兼性厌氧革兰氏阴性菌，通过脱羧、羟基化和氢过氧化机制分解不饱和脂肪酸。